Usion: Medin and MFGE8 are abundant in aged subjects and are secreted by exosomes in to the ECM. Exosome release is elevated with age, which could contribute to the deposition of medin in the ECM and also the formation of amyloid. MFGE8 might play a role in accelerating calcification by inducing an osteogenic phenotype by means of the ERK pathway. Each MFGE8 and medin secretion by exosomes could contribute to the age-related improvement of vascular calcification. Funding: This operate is funded by the British Heart Foundation.made use of as cellular ageing model. Dx accelerated ageing, but Wnt4-containing exosomes could efficiently counteract Dx-induced senescence. We have obtained diverse staining patterns utilizing DiI-labelled Wn4-exosomes on sections of young and aged samples. Finally, in vivo injected DiI-labelled Wnt4-exosomes showed detectable homing towards the thymus. Summary/Conclusion: As outlined by our final results Wnt4 and miR27b are present in TEC exosomes. Our findings indicate that Wnt4 is actually a key inhibitor thymic involution potentially via miR27b. Even so, further experiments are required for achievable applications. Funding: Scientific study support was provided by PTE AOK KA2016-16, PTE Pharmaceutical Talent Center system along with the PTE Viral Pathogenesis Talent Center program by way of KK. The Janos Bolyai Scholarship with the Hungarian Academy of Sciences also supported KK.PS06.Extracellular vesicles and their miRNA cargo in ageing and ageassociated diseases Lucia Terlecki-Zaniewicz1; Vera Pils1; Ingo L mermann1; Regina Weinm lner1; Madhusudan Bobbili Reddy1; Markus Schosserer1; Florian Bcl-2 Inhibitor Accession Gruber2; Matthias Hackl3; HDAC11 Inhibitor manufacturer Johannes Grillari1 CDL for Biotechnology of Skin Aging BOKU Division of Biotechnology, Vienna, Austria; 2Department of Dermatology, Medical University of Vienna, Austria; Christian Doppler Laboratory for the Biotechnology of Skin Aging, Vienna, Austria, Vienna, Austria; 3TAmiRNA GmbH Vienna, Vienna, AustriaPS06.11 = OWP1.Part of Wnt4 exosomes in thymic ageing Krisztina Banfai1; Kitti Garai1; David Ernszt2; Judit E. Pongracz1; Krisztian KvellInstitute of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Pecs, Hungary, P s, Hungary; 2Institute of Physiology, Faculty of Medicine, University of Pecs, Pecs, Hungary, P s, HungaryBackground: Wnt4 plays a crucial function in promoting the development and halting the ageing of the thymus. In the course of ageing Wnt4 is downregulated, whilst PPAR is up-regulated and triggers adipose involution. Even so, miR27b was described to suppress PPAR. Our objective was to prove the presence of Wnt4 in exosomes, to detect its impact and stick to its path each in vitro and in vivo. Procedures: Exosomes were harvested from manage and Wnt4 overexpressing TECs (thymic epithelial cells) for additional experiments. Exosomes had been visualized by transmission electron microscopy. Exosomal miR27b levels have been measured by TaqMan qPCR, whilst Wnt4 protein content was assayed by ELISA. DiI-labelled exosomes had been applied on mouse and human thymus sections as well as iv-injected into mouse for in vivo tracking. Outcomes: Transmission electron microscopy showed exosomes ranging 50100 nm in size. TaqMan miRNA assay measured elevated miR27b levels, although ELISA showed higher Wnt4-content in Wnt4-exosomes when compared with handle exosomes. For functional studies steroid (Dx)-induced TECs wereBackground: Cellular senescence has evolved from an in vitro model method to study ageing to a multifaceted phenomenon of in vivo importance as senescent cell removal delays t.