D by Western blot. Benefits: The MTT assay indicated that the PAEs were not cytotoxic when the concentrations were reduced than 600 /mL. The 25 ethanolic extract of PAE (PRA:AMR/1:1, w/w) Flurbiprofen axetil Cancer dose-dependently decreased the NO production in LPS-stimulated RAW 264.7 cells and using the most potency than other PAEs. The western blot evaluation indicated that the optimized PAE could efficiently inhibit the LPS-induced iNOS expression but not impact the expression of COX-2. Additionally, PAE drastically inhibited LPS-induced activation of NF-B signalling but didn’t influence around the LPS-induced activation of MAPK signaling. Conclusions: PAE selectively inhibits the expression of iNOS by means of suppression of NF-B pathways in inflamed macrophages. Such a preferential suppression cascade by PAEs may have therapeutic prospective for inflammatory diseases with over activation of iNOS. Acknowledgements: This study was financially supported by the opening fund of your State Key Laboratory of Good quality Investigation in Chinese Medicine of Maleimide Autophagy University of Macau (No. SKL-QRCM-2014-2016) and also the Investigation Committee from the University of Macau (SRG2015-00060-ICMS-QRCM).Fig. 1 Distribution of diverse sort of compounds in HS species. SO: S. orientalis L., SP: S. pubescens Makino, SG: S. glabrescens MakinoFig. 2 Main carbonskeleton of kauranetype (A) and pimaranetype (B) diterpenoids in SHsChin Med 2018, 13(Suppl 1):Web page 62 ofSH-related diterpenoids. The key differences of chemical distributions may well to be connected for the sesquiterpenoids. Most germacranolides were located in SO, while a cluster of cadinane sesquiterpenoids had been only identified in SP. Acknowledgements: This study was financially supported by the opening fund with the State Important Laboratory of Good quality Analysis in Chinese Medicine of University of Macau (No. SKL-QRCM-2014-2016), the Investigation Committee of your University of Macau (SRG2015-00060ICMS-QRCM) and the National Organic Science Foundation of China (NSFC, No. 81470170).References 1. Jeon CM, Shin IS, Shin NR, Hong JM, Kwon OK, Kim HS, Oh SR, Myung PK, Ahn KS. Int Immunopharmacol. 2014;22(2):414?. two. Kim HM, Lee JH, Won JH, Park EJ, Chae HJ, Kim HR, Kim CH, Baek SH. Phytother Res. 2001;15(7):572?. three. Lee K, Jung J, Yang G, Ham I, Bu Y, Kim H, Choi HY. Phytother Res. 2013;27(9):1308?2. four. Wang JP, Ruan JL, Cai YL, Luo Q, Xu H X, Wu YX. J Ethnopharmacol. 2011;134(three):1033?. 5. Chang CC, Hsu HF, Huang KH, Wu JM, Kuo SM, Ling XH, Houng JY. Mol ecules. 2014;19(12):19980?four. six. Commission CP. Pharmacopoeia of People’s Republic of China. 2015.Acknowledgements: This research was financially supported by the Start-up Research Grant from University of Macau (SRG2015-00061ICMS-QRCM), along with the opening fund of the State Crucial Laboratory of Good quality Research in Chinese Medicine of University of Macau (No. SKL-QRCM-2014-2016).References 1. Xiao JB, Ni XL, Kai GY, et al. Crit Rev Food Sci Nutr. 2015;55:16?1. 2. Xiao JB, H ger P. Curr Med Chem. 2015;22:23?8. 3. Xiao JB, Ni XL, Kai GY, et al. Crit Rev Meals Sci Nutr. 2013;53:497?06. 4. Xiao JB, Kai GY, Yamamoto K, et al. Crit Rev Meals Sci Nutr. 2013;53:818?6.142 Flavonoid glycosylation and human overall health Hui Cao, Yitao Wang, Jianbo Xiao Institute of Chinese Medical Sciences, State Essential Laboratory of High quality Investigation in Chinese Medicine, University of Macau, Taipa, Macau, China Correspondence: Jianbo Xiao [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):142 Background: The dietary flavonoids, particularly their glycosides, are the most abun.