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Jiang et al. Journal of Experimental Clinical Cancer Research (2017) 36:131 DOI 10.1186/s13046-017-0602-RESEARCHOpen AccessmiR-19b-3p promotes colon cancer proliferation and oxaliplatin-based chemoresistance by targeting SMAD4: validation by bioinformatics and experimental analysesTao Jiang1, Ling Ye2, Zhongbo Han3, Yuan Liu2, Yinxue Yang1, Zhihai Peng2 and Junwei Fan2AbstractBackground: As a illness with particularly complicated molecular mechanisms, a lot of deregulated miRNAs have already been identified in colon cancer. Couple of research have been performed by utilizing Ingenuity Pathways Analysis (IPA) to predict miRNAs specifically expressed in colon cancer. Methods: A characteristic microRNA-target network of colon cancer was explored making use of IPA. Then the clinical significance of miR-19b-3p was evaluated in 211 colon cancer individuals. The roles of miR-19b-3p and its candidate target gene, SMAD4, in colon cancer progression have been examined each in vitro and in vivo. Benefits: Bioinformatics evaluation showed that 15 microRNAs screened by IPA have been significantly correlated with malignant biological behaviors of colon cancer. miR-19b-3p was probably the most considerably HSPA5/GRP-78 Protein Species upregulated candidate determined by the validation experiment working with 211 colon cancer samples. Higher expression of miR-19b-3p was considerably associated with higher N stage (P sirtuininhibitor 0.001), higher AJCC stage (P sirtuininhibitor 0.001), poor histologic grade (P = 0.032), frequent venous and lymphatic invasion (P = 0.027), and liver metastasis (P sirtuininhibitor 0.001). Survival evaluation revealed that miR-19b-3p was an independent prognostic element associated with colon cancer Adiponectin/Acrp30, Mouse (227a.a) patient’s overall survival (OS) and disease-free survival (DFS). miR-19b-3p promoted proliferation and chemoresistance of colon cancer cells, but had no effect on invasion in vitro, as well as tumorigenesis in vivo. Furthermore, we confirmed that miR-19b-3p mediates resistance to oxaliplatin-based chemotherapy through SMAD4. Conclusions: Our findings demonstrate the function of miR-19b-3p-SMAD4 axis in colon cancer progression, which may possibly turn out to be a potential therapeutic target against chemotherapy resistance. Key phrases: miR-19b-3p, SMAD4, Proliferation, Oxaliplatin, Chemoresistance Correspondence: 18895007660@163; pengzhihai1958@163; drjunweifan@163 Equal contributors 1 Division of Anal-Colorectal Surgery, Common Hospital of Ningxia Medical University, Yinchuan 750004, People’s Republic of China 2 Division of Common Surgery, Shanghai Common Hospital, Shanghai Jiao Tong University College of Medicine, Shanghai 20080, People’s Republic of China Full list of author information is readily available in the end of your articlesirtuininhibitorThe Author(s). 2017 Open Access T.