E = 4.4) and CSAM (detected only in CRC tissue), considering the fact that their expression levels in plasma EVs from CRC patients have been also considerably greater than these from healthful donors in EV-ELISA assays employing independent validation set. IHC staining evaluation also demonstrated 4 EV proteins especially overexpressed in CRC cells. Interestingly, uptake of STAM++ EVs enhanced both proliferation and invasion of recipient cells in vitro. Conclusions:As a result TMAM, STAM, GAM and CSAM on EVs should be Ubiquitin-Conjugating Enzyme E2 D1 Proteins Recombinant Proteins potential diagnostic or prognostic biomarkers for CRC, leading to development of precise, non-invasive and low-cost blood liquid biopsy tests in future.Saturday, May well 20,Area: Harbour Ballroom Symposium Session 24 EV Functions in Inflammation Chairs: Saara Laitinen and Takahiro Ochiya 1:30:00 p.m.OS24.Extracellular vesicles from adipose-derived mesenchymal stem cells promote autophagy in human osteoarthritic chondrocytes Miguel Tofi -Vian1, Maria Jose Alcaraz1, Maria Dolores Perez del Caz2, Miguel Angel Castejon3 and Isabel Autophagy-Related Protein 3 (ATG3) Proteins Storage & Stability Guillen4 IDM, University of Valencia; 2Department of Burn and Plastic Surgery, La Fe University Hospital; 3Department Orthopaedic Surgery and Traumatology, La Ribera University Hospital; 4IDM, University of Valencia and Department of Pharmacy, CEU-Cardenal Herrera, ValenciaIntroduction: Adipose tissue-derived mesenchymal stem cells (ADMSC) release extracellular vesicles (EV) both beneath physiological and pathological circumstances. The immunomodulatory and anti-inflammatory properties of AD-MSC have proven to be effective in several illnesses. Osteoarthritis (OA) can be a top reason for disability inside the elderly. Cartilage destruction is mediated by alterations in chondrocyte metabolism plus the up-regulation of inflammatory or catabolic genes. In OA chondrocytes, the induction of autophagy may perhaps be a protective mechanism against pressure. We’ve investigated the effects of microvesicles (MV) and exosomes (EX) from AD-MSC on autophagy, measured as LC3Bpositive autophagosome formation, along with the production of inflammatory and catabolic mediators in OA chondrocytes stimulated with IL-1. Techniques: AD-MSC have been isolated from fat of individuals who undergone abdominoplasty. EV have been isolated from AD-MSC conditioned medium by differential centrifugation with size filtration. Tunable resistive pulse sensing was made use of to evaluate the concentration and size of Ex and Mv. OA chondrocytes have been stimulated with IL-1 (ten ng/mL) and treated with MV (3.six 107 particles/mL) or EX (7.2 107 particles/mL) for 24 h. The levels of oxidised proteins, IL-6, IL-10 and TNF were measured by ELISA, PGE2 by RIA, and MMP activity and NO by fluorometry. The expression of collagen II and LC3B was evaluated by confocal microscopy. The data have been analysed by ANOVA followed by Dunnett’s test. Results and Conclusion: EV down-regulated the production of inflammatory and degradative mediators induced by IL-1. Treatment of OA chondrocytes with MV or EX resulted within a significant reduction of MMP activity, oxidative tension, IL-6 and TNF levels. Also, they enhanced the production with the anti-inflammatory cytokine IL-10 as well as the expression of collagen II. Each varieties of EV promoted the liberation of LC3B-positive autophagosomes, having a larger impact for MV. Our information indicate that EV exert protective effects on OA chondrocytes and may possibly have prospective pharmacological applications to control autophagy, inflammatory processes and extracellular matrix degradation. Funding: SAF2013-48724-R (MINECO, FEDER).