journal.pone.0054135

GITR/TNFRSF18 Antibody (AP-MAB0860) Summary

    Immunogen
    mouse CD25+CD4+ T cells
    Specificity
    This was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse CD25+CD4+ T cells.
    Isotype
    IgG2
    Clonality
    Monoclonal
    Host
    Rat
    Gene
    TNFRSF18
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Flow Cytometry 1:10-1:1000
        CyTOF-ready
    Application Notes
    Flow Cytometry and Blocking mouse GITR (in vitro and in vivo). Extremely low level of LPS (< 0.002EU/ug IgG)

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    A 0.2 um filtered solution in PBS – Azide free
    Preservative
    No Preservative
    Concentration
    1.0 mg/ml
    Purity
    Protein A or G purified

Alternate Names for GITR/TNFRSF18 Antibody (AP-MAB0860)

      Activation-inducible TNFR family receptor
      AITR
      AITRTNF receptor superfamily activation-inducible protein
      CD357 antigen
      CD357
      GITR
      GITR-D
      GITRtumor necrosis factor receptor superfamily member 18
      Glucocorticoid-induced TNFR-related protein
      TNFRSF18
      tumor necrosis factor receptor superfamily, member 18

Background

GITR, glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25+CD4+Treg cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+CD4+ T reg cells. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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