fpubh.2014.00130

Caspase-6 Antibody (6CSP01 (7.1.65)) Summary

    Immunogen
    Recombinant human Caspase 6 prodomain.
    Epitope
    Caspase 6 prodomain.
    Localization
    Cytoplasmic.
    Specificity
    Caspase 6 (6CSP01 (7.1.65))
    Isotype
    IgG1 Kappa
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    CASP6
    Purity
    Protein A or G purified
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Applications/Dilutions

    Dilutions
        Immunoprecipitation 2ug/mg
    Application Notes
    Immunoprecipitation (denatured verified, with protein G): use at a concentration of 2ug/mg of lysate. Not tested in any other applications. Optimal dilutions/concentrations should be determined by the end user.
    Positive Control Caspase-6 Lysate (NBL1-08715)

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Buffer
    10mM PBS (pH 7.4) and 0.2% BSA
    Preservative
    0.09% Sodium Azide
    Purity
    Protein A or G purified

Alternate Names for Caspase-6 Antibody (6CSP01 (7.1.65))

      Apoptotic protease Mch-2
      CASP6
      CASP-6
      caspase 6, apoptosis-related cysteine peptidase
      caspase 6, apoptosis-related cysteine protease
      Caspase6
      Caspase-6
      EC 3.4.22
      Mch2
      MCH2EC 3.4.22.59

Background

Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

NVP-BGJ398

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