CD15/Lewis X Antibody (C3D-1) Summary
Purified neutrophils from normal human peripheral blood.
Cell membrane
The specificity of this antibody is similar to antibodies Leu-M1, TU 9 and VIM-D5, recognizing an epitope involving the carbohydrate sequence 3-fucosyl-N-acetyllacetosamine. This antibody reacts with an antigen located on mature granulocytes and on Hodgkin and Reed-Sternberg cells.
IgM Kappa
Monoclonal
Mouse
FUT4
Tissue culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
Applications/Dilutions
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:25
IHC-Paraffin requires high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 or Tris-EDTA buffer, pH 9.0 prior to immunostaining.
Reactivity Notes
Human.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
No buffer
Sodium Azide
Tissue culture supernatant
Alternate Names for CD15/Lewis X Antibody (C3D-1)
- CD 15
- CD15
- CD-15
- Lewis X
- Lewis-X
- LeX
- SSEA-1
- stage-specific embryonic antigen 1
Background
The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkins disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.