BRCA1 Antibody (GLK-2) Summary
Synthetic peptide: LDSVALYQCQELDTYLIPQIPHSHY, corresponding to C terminal amino acids 1839-1863 of Human BRCA1.
Cytoplasmic
Stains 94% of ovarian carcinomas with a mutation in exon 11. However, staining is absent in 100% of tumors with mutations other than exon 11.
IgM
Monoclonal
Mouse
BRCA1
Tissue culture supernatant
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Applications/Dilutions
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
IHC-P: 1/15 – 1/50 in an ABC method. An incubation period of 30-60 minutes at room temperature is recommended. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Reactivity Notes
Cross-reacts with Human. Expected to cross-react with Cow (94% identity with immunogen), Chimpanzee (100% identity with immunogen), Dog (100% identity with immunogen), Gorilla (100% identity with immunogen) and Rhesus monkey (100% identity with immunogen) due to sequence homology. Not yet tested in other species.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No buffer
Sodium Azide
Tissue culture supernatant
Alternate Names for BRCA1 Antibody (GLK-2)
- BRCA1
- BRCAI
- breast and ovarian cancer susceptibility protein 1
- breast and ovarian cancer sususceptibility protein
- breast cancer 1, early onset
- breast cancer type 1 susceptibility protein
- EC 6.3.2
- EC 6.3.2.-
- IRIS
- PNCA4
- PSCP
- subunit 1
Background
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.