The H2O2dependent oxidation of ascorbate was followed by checking the reduce in absorbance at 290 nm, assuming an absorption coefficient of two.8 mM cm
Tissue sections had been incubated with ten mM Alexa fluor 488 Hg-url phenylmercury (Molecular Probes, cat. no H30462) at 25uC for two h, in darkness, and then had been washed…