The adhering to cycling protocol was utilized: denaturation at 95uC for 2 min and amplification at 95uC for fifteen sec, 55uC for thirty sec and 72uC for one min for 36 cycles
In Southern blot analysis, fifteen micrograms of sheep genomic DNA have been isolated as explained in other places and digested with restriction enzymes Bcl I, EcoR I or Hinc II…