s12015-015-9626-4

Neutrophil Elastase/ELA2 Antibody (4B11) Summary

    Immunogen
    ELA2 (NP_001963 168 a.a. – 267 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH
    Specificity
    ELA2 (4B11)
    Isotype
    IgG2a Kappa
    Clonality
    Monoclonal
    Host
    Mouse
    Gene
    ELANE
    Purity
    IgG purified
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Applications/Dilutions

    Dilutions
        ELISA
    Application Notes
    Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    PBS (pH 7.4)
    Preservative
    No Preservative
    Purity
    IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Neutrophil Elastase/ELA2 Antibody (4B11)

      Bone marrow serine protease
      EC 3.4.21
      EC 3.4.21.37
      ELA2
      ELA2granulocyte-derived elastase
      ELANE
      elastase 2, neutrophil
      elastase, neutrophil expressed
      Elastase-2
      GE
      HLEelastase-2
      HNE
      Human leukocyte elastase
      Leukocyte Elastase
      Medullasin
      NE
      Neutrophil Elastase
      PMN elastase
      PMN-E
      polymorphonuclear elastase
      SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Elastase 2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the proteins release from activated neutrophils. Elastase 2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

product targets : MAPK_Compound_Library inhibitors

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