14651858.CD004794.pub3

Human Apolipoprotein A5 ELISA Kit (Colorimetric) Summary

    Specificity
    This assay has high sensitivity and excellent specificity for detection of human Apo-A5. No significant cross-reactivity or interference between human Apo-A5 and analogues was observed.
    Standard Curve Range
    31.2 pg/ml-2000 pg/ml
    Sensitivity
    7.8 pg/ml
    Assay Type
    Competitive ELISA
    Inter-Assay
    CV%<10%
    Intra-Assay
    CV%<8%
    Sample Volume
    50-100ul
    Kit Type
    ELISA Kit (Colorimetric)
    Gene
    APOA5

Packaging, Storage & Formulations

    Storage
    Store the unopened product at 2 – 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
      Standard (Freeze dried)
      Sample Diluent
      Stop Solution
      TMB Substrate
      Wash Buffer (25 x concentrate)
      Assay plate
      Adhesive Strip (For 96 wells)
      Instruction manual
      HRP-conjugate (100 x concentrate)
      HRP-conjugate Diluent

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Human Apolipoprotein A5 ELISA Kit (Colorimetric)

      APOAV
      apo-AV
      APOA-V
      Apolipoprotein A5
      apolipoprotein A-V
      FLJ97995
      MGC126836
      RAP3MGC126838
      Regeneration-associated protein 3

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-A5. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-A5. The competitive inhibition reaction is launched between with pre-coated Apo-A5 and Apo-A5 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-A5 in the samples. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

product targets : GPR120 inhibitors

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