Pecifically in contrast to antibodies. Using the CuAAC technology, baseclick has prepared aptamers with two different binding specificities combined in a single aptamer molecule by efficiently joining two aptamers. These aptamers have similar applications as bi-specific antibodies and thus could bring together two different cells (e.g., cancer cell and T cell) and induce targeted cancer cell destruction.
CONCLUSION Other applications of the CuAAC that have been developed within the group of Prof. Carell in recent years include synthesis of labelled nucleotides,[7] and synthesis of probes for the sequencing of epigenetic markers.[8] Despite the numerous applications which have already been developed using CuAAC, many more are expected in the near future. Using new
baseclick GmbH has been granted the following patents (1-3) besides its further patent applications (4-5). 1. WO 2006/117161 (New labelling strategies for the sensitive detection of analytes) 2.61849-14-7 InChIKey WO 2008/952775 (Click chemistry for the production of reporter molecules) 3. WO 2010/115957 (Click Chemistry on heterogeneous catalysts) 4.347841-88-7 Description PCT/EP 2013/064610 (Anandamide-modified nucleic molecules) 5.PMID:30137786 PCT/EP 2015/056007 (Self-assembly of DNA Origami: a diagnostic tool) baseclick GmbH holds a worldwide exclusive license for granted patent application WO 03/101972 (Copper-catalysed ligation of azides and acetylenes for the nucleic acid field) in the area of diagnostics and research. As Glen Research and baseclick are partners, Glen Research is now able to help in sublicensing this outstanding technology.
First introduced in 2002, Copper(I)catalyzed azide-alkyne cycloaddition (CuAAC) is a robust and versatile Click Chemistry methodology commonly characterized by high yields, simple conditions, mild reagents, innocuous by-products, and simple purification.1-3 Subsequent developments by Carell and Seela with copper-stabilizing ligands that protect DNA from copper-induced damage and alkyne-bearing modifications for oligonucleotide synthesis have produced a versatile method that is fully compatible with oligonucleotide labelling.4-8 In 2010, Glen Research partnered with baseclick GmbH to offer alkyne-bearing phosphoramidites, azides, and ancillary reagents for labelling oligonucleotides using CuAAC Click conjugations. Through an updated agreement with baseclick we are pleased to continue to offer and expand our list of these versatile reagents for labelling of oligonucleotides for research and development use. Previous work incorporating 5-Ethynyl-dU-CE Phosphoramidite (1) in oligonucleotide synthesis showed that this
modified nucleoside worked well, albeit with some restrictions. Unlike the C8-Alkyne-dTCE Phosphoramidite (2), 5-ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat.9 Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions. Mild deprotection conditions are necessary to prevent this side reaction. Acid catalyzed reactions are also possible for 5-ethynyl-dU although we did not observe any degradation in our short test oligos. In this article, we introduce TIPS5-Ethynyl-dU-CE Phosphoramidite (3). This protected alkyne offers broader compatibility with oligonucleotide synthesis and deprotection. Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevented acid or base catalyzed hydration during oligonucleotide synthesis and workup.9 A q.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com