Tween the four periods (p > 0.05 repeated measures ANOVA)(Table 2), whereas serum
Tween the four periods (p > 0.05 repeated measures ANOVA)(Table 2), whereas serum C3 and ceruloplasmin levels were significantly reduced during the albumin period (p < 0.05 non parametric ANOVA, Friedman test followed by Dunn's post test)(Figure 2).Table 2 Inflammatory parameters during albumin and gelatin infusionsParameter CRP(mg/l) Ceruloplasmin (g/l) C3 (g/l) Haptoglobin (g/l) Cyclopamine supplier before treatment 7.12 ?8.01 0.22 [0.14 – 0.34] 0.97 [0.72-1.59] 1.35 ?0.41 Albumin infusion period 9.25 ?11.02 0.19 [0.13-0.34] 0.78 [0.61-1.29] 1.25 ?0.50 Wah-Out period 6.46 ?7.10 0.23 [0.16 – 0.32] 0.96 [0.71-1.40] 1.22 ?0.43 Gelatin infusion period 4.78 ?4.70 0.23 [0.16-0.33] 0.96 [0.69-1.39] 1.31 ?0.41 Statistical analyses p > 0.05 at the repeated measures ANOVA p < 0.05 at the Friedman test p < 0.05 at the Friedman test p > 0.05 at the repeated mesures ANOVARostoker et al. BMC Nephrology 2011, 12:58 http://www.biomedcentral.com/1471-2369/12/Page 5 ofFigure 2 Concentration of serum ceruloplasmin (g/l) in the whole group (n = 10).Oxidative stressPlasma total antioxidant power did not differ between the four periods (p > 0.05 repeated measures ANOVA)(Table 3). Serum hydrogen peroxide values differed between the four periods (Table 3 and figure 3) (p < 0.01 repeated measures ANOVA) and were significantly lowered during the albumin period, wash-out period and gelatin period (p < 0.01 Dunnett's multiple comparisons test)(Figure 3). Serum lipid peroxides were strongly and significantly reduced only during the albumin period (p < 0.01 non parametric ANOVA Friedman test followed by Dunn's post-test) (Table 3 and figure 4). Nitrotyrosine was undetectable at PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28499442 the beginning of the dialysis sessions during the four periods (data not shown). Serum lactoferrin levels before and after dialysis sessions did not differ between the four periods (p > 0.05 repeated measures ANOVA)(Table 3).Serum cytokinesprotocol (data not shown). Serum levels of the proinflammatory cytokines IL6, IL8 and TNF alpha did not differ between the four periods of the protocol at the beginning or end of the dialysis sessions (p > 0.05 repeated measures ANOVA)(Table 3).Serum nitrite and nitrate levelsThe sum of serum nitrite and nitrate levels did not differ between the four periods of the protocol at the beginning or end of the dialysis sessions (p > 0.05 repeated measures ANOVA)(Table 3).IL-1 beta was undetectable both at the beginning and end of the dialysis sessions during each period of theDiscussion We recently showed in a pilot single-blind cross-over study, using n-of-1 trial methodology, that systematic infusion of 20 albumin or 4 gelatin during hemodialysis sessions in hypotension-prone patients unresponsive to preventive measures improves hemodynamic parameters (systolic and diastolic blood pressure), increases the ultrafiltration rate and reduces the numberRostoker et al. BMC Nephrology 2011, 12:58 http://www.biomedcentral.com/1471-2369/12/Page 6 ofTable 3 Oxidative stress, serum cytokines, serum nitrites and nitrates during albumin and gelatin infusionsParameter Plasma total anti-oxydant power (copper reducing equivalent microM) Hydrogen peroxyde g/ml Lipid peroxides (H202 equivalent) M/1 Lactoferrin at the beginning of the dialysis session (ng/ml) Lactoferrin at the end of the dialysis session (ng/ml) IL6 at the beginning of the dialysis session (pg/ml) IL6 at the end of the dialysis session (pg/ml) IL8 at the beginning of the dialysis session (pg/ml) TNF a at the beginning of the dia.