Etc. (stages were annotated at the first frame in which the
Etc. (stages were annotated at the first frame in which the cells were seen as separated by membranes); tM – the time of the first frame in which the embryos were compacting into the morula stage; tB – the time of the frame in which a crescent-shaped area began to emerge from the morula;Wdowiak and Bojar Reproductive Health (2015) 12:Page 4 ofand tEB ?the time of the frame with expanded blastocyst with increased volume and expansion of the blastocoele cavity. Based on the analysis of time-lapse records and in accordance with American Society for Reproductive Medicine and the European Society of Human Reproduction and Embryology consortium guidelines [15], a single blastocyst was selected to be transferred into the uterus. After 6 weeks, the presence of the embryo and its cardiac activity were order 4-Hydroxytamoxifen assessed via ultrasound.Determination of hormone levels and imaging studiesstudy and signed a written consent allowing the use of their data for research purposes.ResultsCharacteristics of the studied population and the effectiveness of IVFLevels of FSH, LH, and AMH were determined on the third day of the cycle preceding ovulation, before administration of drugs. Ultrasound examination was performed daily since 9th day of cycle and after observation of a follicle with dimension equal to or greater than 17 mm; daily measurement of estradiol levels was implemented. The estradiol values were then converted into the levels of estradiol per follicle equal to or greater than 17 mm (E2/f ). All hormones levels were measured in serum obtained from morning blood samples (5 mL). Levels of FSH, LH, and estradiol were assessed with electrochemiluminescent method on a Cobas analyzer (Roche Diagnostics) ?the reference values were: for FSH 3.5?2.5 mIU/mL; for LH 2.4?2.6 mIU/mL; and for estradiol 12.5?66 pg/mL. Levels of AMH were measured with AMH Gen II ELISA test (Beckman Coulter) on a Euroimmun analyzer (reference range > 1.5 ng/mL).Statistical analysisThe mean age of the women included in the study was 30.90 ?2.93 years and ranged from 25 to 35 years. In turn, the BMI of the studied women was 22.88 ?3.27 kg/m2 and ranged from 17 to 30 kg/m2. The women were divided into two groups according to the results of IVF. Group A consisted of 77 women (34.8 ) who achieved pregnancy, and group B was made up of 144 women (65.2 ) in whom pregnancy has not been achieved. In group A, 42 women (54.55 ) were stimulated with hFSH and 35 women (45.45 ) with rFSH. In group B, hFSH was administered to 73 women (50.69 ) and rFSH to 71 women (49.31 ) (Fig. 2). No statistically significant difference was found between the two groups with regards PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27488460 to the type of gonadotropins used (Chi2 = 0.298, df = 1, p = 0.585). Detailed information about the studied population is presented in Table 1.Time-lapse evaluationThe obtained results were subjected to the Statistica 9.1 software system (StatSoft, Poland). The measurable parameters were reported as mean (M), standard deviation (SD), and minimum (Min) or maximum (Max) whereas the values of the immeasurable parameters were reported as proportion and quantity. For quality attributes, the Chi2 test was performed to show differences between the examined groups. The Shapiro-Wilk normality test was used to check the normality of distribution of variables in the examined PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27488460 groups. The Mann-Whitney Utest was carried out to examine differences between the groups. The r-Pearson correlation test was used to check the correlations between variables.