Help you at every step:?We accept pre-submission inquiries ?Our selector
Help you at every step:?We accept pre-submission inquiries ?Our selector tool helps you to find the most relevant journal ?We provide round the clock customer support ?Convenient online submission ?Thorough peer review ?Inclusion in PubMed and all major indexing services ?Maximum visibility for your research Submit your manuscript at www.biomedcentral.com/submit
Perrini et al. Stem Cell Research Therapy (2016) 7:169 DOI 10.1186/s13287-016-0429-RESEARCHOpen AccessMicrovesicles secreted from equine amniotic-derived cells and their potential role in reducing inflammation in endometrial cells in an in-vitro modelClaudia Perrini1, Maria Giuseppina Strillacci2, Alessandro Bagnato2, Paola Esposti1, Maria Giovanna Marini3, Bruna Corradetti3, Davide Bizzaro3, Antonella Idda4, Sergio Ledda4, Emanuele Capra5, Flavia Pizzi5, Anna Lange-Consiglio1* and Fausto Cremonesi1,AbstractBackground: It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication. Methods: In this context, this study aims to understand the efficacy of MVs in in-vitro endometrial stressed cells in view of potential healing in in-vivo studies. For this purpose, the presence and type of MVs secreted by amniotic mesenchymal stem cells (AMCs) were investigated and the response of endometrial cells to MVs was studied using a dose-response curve at different concentrations and times. Moreover, the ability of MVs to counteract the in vitro stress in endometrial cells induced by lipopolysaccharide was studied by measuring the rate of apoptosis and cell proliferation, the expression of some pro-inflammatory genes such as tumor necrosis factor- (TNF-), interleukin-6 (IL-6), interleukin 1 (IL-1), and metalloproteinases (MMP) 1 and 13, and the release of some pro- or anti-inflammatory PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28192408 cytokines. Results: MVs secreted by the AMCs ranged in size from 100 to 200 nm. The incorporation of MVs was gradual over time and peaked at 72 h. MVs reduced the apoptosis rate, increased cell proliferation values, downregulated pro-inflammatory gene expression, and decreased the secretion of pro-inflammatory cytokines. Conclusion: Our data suggest that some microRNAs could contribute to counteracting in-vivo inflammation of endometrial tissue. Keywords: Microvesicles, Endometrium, Inflammation, LPS, Regenerative medicineBackground The regular uterine environment promotes normal embryo development, but clinical or subclinical disorders could contribute to pregnancy failure. As reviewed by Hurtgen [1], endometritis is an important cause of reduced fertility in mares in which artificial insemination by fresh or frozen semen may induce acute endometrial inflammatory Cyclosporine supplier reactions. If these conditions are not promptly resolved, infections become chronic and, in old pregnant mares, often result in higher pregnancy* Correspondence: [email protected] 1 Large Animal Hospital, Reproduction Unit, Universit?degli Studi di Milano, Via dell’Universit?6, 26900 Lodi, Italy Full list of author information is available at the end of the articleloss. A similar clinical endometritis also occurs in dairy cows following parturition [2, 3]. Furthermore, cytological endometritis emerged as a problem of remarkable importance for PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27797473 dairy cattle reproduction because animals suffering from this disorder present a persistent inflammatory uterine environment even in the absence of clinical symptoms. A.