Rabbit anti-TOP2α Polyclonal Antibody

Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym Topoisomerase Ⅱalpha; DNA topoisomerase II; alpha isozyme; DNA topoisomerase 2 alpha; DNA topoisomerase II 170 kD; DNA Topoisomerase2; TOP2; TOP2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TP2A; TPⅡA; TOP2A_HUMAN; Topo IIα; Topo-IIα |Host Rabbit |Specificity TOP2A |Species Reactivity Rat, |Predicted Reactivity Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit |Applications ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |Immunogen KLH conjugated synthetic peptide derived from human TOP2 alpha:551-650/1531 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Tumor Chromatin and nuclear signaling Signal transduction Transcriptional regulators |Target |Background This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]. |Cellular localization Cell nucleus; Cytoplasm; |UniProt P11388 | Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (TOP2A) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.