And outcome were entered in a pro forma. Results: Out of
And outcome were entered in a pro forma. Results: Out of 3,142 ICU admissions, the incidence of candidemia was 29.6/1,000 admissions. A high proportion of patients (80.6 ) had an indwelling urinary catheter with the mean duration of catheter days being 5.89 ?3 days. Other associated risk factors such as diabetes mellitus and antibiotic usage were seen in 74.2 and 86 , respectively. Concomitant candidemia was seen in 19.4 of cases. Nonalbicans Candida spp. (66.7 ) emerged as the predominant pathogen causing candiduria. ICU mortality was 29 (Table 1). Conclusion: There is a high incidence of candiduria in ICU patients, especially among those with indwelling catheters and those on antibiotic therapy. Moreover, in our cohort, an increased proportion of patients with candiduria had noncandida infection, emphasizing the need to have localized regional surveillance studies to identify the locally prevalent candida species and devise antifungal therapy protocols.P20 Erythropoietin enhances the effects of transplanted mesenchymal stem cells in an experimental model of endotoxemia A Sorokina1*, A Averyanov1, F Zabozlaev1, A Konoplyannikov2, D Akulshin1, O Kuzovlev1 1 Federal Research Clinical Center FMBA of Russia, Moscow, Russia; 2 Radiologic Research Center, Obninsk, Russia Critical Care 2012, 16(Suppl 3):P20 Background: Mesenchymal stem cells (MSCs) are able to reduce systemic inflammatory response in an experimental sepsis. One of the limiting factors of MSC therapy is a high degree of apoptosis of transplanted cells. On the basis of recently detected receptors for erythropoietin (EPO) on the surface of MSCs we hypothesized that introduction of EPO together with MSCs may increase survival of grafted MSCs and improve the clinical efficacy of cell transplantation. Methods: Fifty Wistar male rats were randomized into five groups with 10 animals in each: Group 1 were healthy controls, Groups 2 to 5 were intraperitoneally introduced bacterial LPS 20 mg/kg. Two hours later LPSCritical Care 2012, Volume 16 Suppl 3 http://ccforum.com/supplements/16/SPage 11 ofTable 1(abstract P20) Morphometric characteristics of the examined tissues in the experimental groupsControls Alveolar septal thickening (M) Kidney tubular dystrophy (point) T-lymphocyte volume in thymus cortex ( ) Spleen white pulp volume ( )*P < 0.05, **P < 0.01 compared with LPS group.LPS 31.25 ?2.9 2.54 ?0.31 34.59 ?1.34 11.15 ?3.LPS + MSCs 15.3 ?2.29* 2.22 ?0.29 49.37 ?2.16 19.9 ?2.LPS + EPO 28.6 ?4.1 1.90 ?0.12* 38.21 ?1.73 22.8 ?0.LPS + EPO +MSCs 10.82 ?1.08** 0.74 ?0.19** 81.48 ?2.63** 27.15 ?2.89*10.32 ?2.05 0 86.24 ?2.88 23.95 ?2.Figure 1(abstract P20) Representative hematoxylin and eosin staining of thymus tissue 96 hours post LPS injection (?00).injection animals received the following intravenous treatment: 4 ?105 allogeneic MSCs (Group 3), 8.5 g PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26100631 recombinant EPO-b (Group 4), MSCs and EPO in the same doses (Group 5). Surviving animals were buy Belinostat euthanized on the fourth day. The morphological study, white blood cell count (WBC) and serum level of IL-1b, IL-2, IL-6, and TNFa measurement were performed. Results: The highest WBC was found in the group of combined treatment EPO + MSCs. The serum IL-1b level in Groups 2 and 4 was significantly higher than in healthy and treated with MSCs and EPO + MSCs animals. The main results of the morphologic analysis of different tissues in the groups are presented in Table 1. The most significant differences in the LPS + EPO group were found in the ly.