G potential score. Briefly, if the VZ score of the longer
G potential score. Briefly, if the VZ score of the longer ORF between the two overlapping ORFs minus a given value is still larger than PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/29045898 that of the shorter one, it is recognized as gene, and the shorter is a non-coding one. Otherwise, both are kept as coding. For more detail, refer to [4].Authors’ contributionsCTZ guided the whole study and took part in writing the manuscript. FBG designed the algorithm and wrote the computer program. He also run the program for about 1000 genomes and took part in writing the manuscript.AcknowledgementsWe thank Dr Ren Zhang for invaluable assistance. We also thank Drs Ju Wang and Ling-Ling Chen for useful discussions. Suggestions from Feng Gao, Yun-Tao Dou and Jian-Hui Zhang on the manuscript are gratefully acknowledged. The present study was supported in part by the National Natural Science Foundation of China (grant 90408028) and the Program of CSIRTU by the Ministry of Education of China.
Soumaya et al. BMC Complementary and Alternative Medicine 2013, 13:28 http://www.biomedcentral.com/1472-6882/13/RESEARCH ARTICLEOpen AccessPharmacological, antioxidant, genotoxic studies and modulation of rat splenocyte functions by Cyperus rotundus extractsKilani-Jaziri Soumaya1,2, Mhalla Dhekra1,2, Ch ane Fadwa1,2, Ghedira Zied1,2, Limem Ilef1,2, Ghedira Kamel1 and Chekir-Ghedira Leila1*AbstractBackground: Cyperus rotundus Linn. (Cyperaceae) is a Tunisian medicinal plant used in folkloric (traditional) medicine to treat stomach disorders and inflammatory diseases. The present PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25645579 study explored the analgesic, anti-inflammatory and genotoxic activities of extracts from the aerial parts of C. rotundus. The antioxidant capacity and the modulation of splenocyte functions by these extracts were also investigated in mice. The phytochemical analysis was carried out using standard methods. Methods: Aqueous, ethyl acetate, methanol and TOF-enriched extracts (300, 150, and 50 g/ml) were evaluated for their analgesic and anti-inflammatory activities. 4, 2, and 1 mg/ml of each extract were tested to investigate their effect on lipid peroxidation. The genotoxic study was monitored by measuring the structural chromosome aberrations of mice treated with 300 mg/kg of extract. The proliferation of lymphocytes in the absence and presence of mitogens was assessed at a concentration range 1?000 g/ml. Results: The tested extracts were able to decrease the mouse ear oedema induced by xylene. purchase AG-490 Furthermore, it was shown that the same extracts reduced the number of abdominal contractions caused by acetic acid in mice, revealing the peripheral analgesic activity of these extracts. It is worth noting that mice treated with doses up to 300 mg/kg b.w. of Cyperus rotundus extracts did not exhibit any toxicity. The tested extracts significantly enhance lymphocyte proliferation at 1 mg/ml. Conclusions: It appears that C. rotundus extracts contain potent components such as flavonoids that may potentially be useful for modulating the immune cell functions, provoking analgesic, anti-inflammatory and antioxidant effects. Keywords: Cyperus rotundus, Analgesic activity, Anti-inflammatory activity, Lipid peroxidation effect, Chromosome aberrations, Immunomodulatory effectBackground Tunisia is a rich source of medicinal plants, and a number of plant derived extracts are used to fight against various diseases. Only few plants have been scientifically explored. Plant derived natural products such as alkaloids, tannins, terpenes and flavonoids have received cons.