Supporting the hypothesis that acetylation of Gli2 is an inhibitory modification, K757Q substitution prevented practically absolutely the Gli-dependent transcriptional activation, irrespective of the stages of the expressed proteins had been equivalent (Fig. 1D)

Sections from paraffin-embedded tissues had been deparaffinized with xylol, fixed with lowering ethanol concentrations, blocked and incubated with anti Gli1 (H-three hundred, 1:two hundred, SantaCruz Biotechnology, SC-20687), or anti acetyl-Gli1(Lys518)…