Name :
Recombinant Rat ADAM17 Protein (His Tag)
Biological Activity :
Background :
ADAM17 is a member of the ADAM protein family of disintegrins and metalloproteases. ADAM17 is ubiquitously expressed in the human colon, with increased activity in the colonic mucosa of patients with ulcerative colitis, a main form of inflammatory bowel disease. The expression of ADAM17 may be inhibited by ethanol. It is involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. ADAM17 also plays a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding.
Biological Activity :
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).The specific activity is >250 pmol/min/µg.
Expression Host :
Rat
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q9Z1K9
NCBI Gene ID :
Synonyms :
Synonyms :
ADAM metallopeptidase domain 17
Amino Acid Sequence :
Molecular Weight :
The recombinant rat ADAM17 comprises 557 amino acids and predicts a molecular mass of 63 kDa. The apparent molecular mass of the recombinant protein is approximately 57 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the rat ADAM17 (Q9Z1K9) (Met1-Asp563) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Poghosyan. et al., 2002, J Biol Chem. 277 (7): 4999-5007. Li Y. et al., 2006, Blood. 108 (7): 2275-9. Peiretti. et al., 2003, J Cell Sci. 116 (10): 1949-57.
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