Epoch Gig Harbor Green (4) and 6-FAM (5)
are based
HARBOR GREENTM PHOSPHORAMIDITE
Epoch Gig Harbor Green (4) and 6-FAM (5)
are based on the same fluorescein core structure. The difference between the two molecules is the linker strategy. Therefore, there should be little difference between Gig Harbor Green and FAM in terms of absorbance and emission spectra. It is well known that the fluorescence of fluoresceinbased dyes depends on the degree of ionization of the phenolic groups and, therefore, is pH dependent. Gig Harbor Green is more pH dependent than FAM, which means that at pH below 7.5 fluorescence will drop slightly faster for Gig Harbor Green than for FAM. On the other hand, at higher pH such as 8.5 (PCR conditions) both dyes are completely ionized and Gig Harbor Green is 15-20% brighter than FAM.6 5′-Aldehyde-Modifier and Gig Harbor Green are offered in collaboration with Epoch Biosciences, Inc. Please read the following statement before choosing to use these products commercially. “These Products are for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Products are subject to proprietary rights of Epoch Biosciences, Inc. and are made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to the Products and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of the Products. “Commercial use” includes but is not limited to the sale, lease, license or other transfer of the Products or any material derived or produced from them, the sale, lease, license or other grant of rights to use the Products or any material derived or produced from them, or the use of the Products to perform services for a fee for third parties (including contract research).”
phosphoramidite in sufficient quantity to offer it for sale. Due to the strategy used to synthesize our version of this phosphoramidite, it has methyl protecting groups on phosphorus, rather than the more usual cyanoethyl.95094-00-1 manufacturer This monomer exhibits high purity, performance and stability but requires the use of thiophenoxide (or another phosphate demethylating reagent) prior to regular deprotection. Cis-syn thymine dimer phosphoramidite (5) is by far the most expensive product we have offered for sale.2754428-18-5 Molecular Weight We are packaging it initially in standard ABI and Expedite vials, as well as amber V vials compatible with the Expedite synthesizer.PMID:29953331 Using this V vial containing 50 micromoles, it is possible to prepare 6 oligos on a 0.2 ole scale each containing a single insertion, or 4 oligos on a 1 ole scale. Similar results should be possible using the LV cycles in Applied Biosystems instruments. Given the high price of this phosphoramidite, if only one or two oligos are required, it makes sense to have a custom oligo house
prepare them. A custom oligo house may also wish to group oligos for several clients for most eficient use of this phosphoramidite. Contact us for the names of custom oligo services active in preparing oligos containing cis-syn thymine dimer. INTRODUCTION
In this issue of The Glen Report, we are introducing a selection of new products and methodologies. One would think that capping in oligonucleotide synthesis would have been developed to the nth degree, but incomplete capping continues to be one of the main contributors to problems. We have, therefore, reviewed the capping efficiency of the current capping mixes and have compared this efficiency achieved to that achieved by.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com