eIF2 alpha/EIF2S1 Overexpression Lysate (Denatured) Summary
Pasmid: pCMV-EIF2S1 full-length
EIF2S1
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).
Lysate Details for eIF2 alpha/EIF2S1
Overexpression
Denatured
Notes
Quality control test: Transient overexpression cell lysate was tested with Anti-EIF2S1 antibody by Western Blots.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for eIF2 alpha/EIF2S1 Overexpression Lysate (Denatured)
- eIF2 alpha
- EIF2
- EIF-2
- EIF2A
- EIF-2A
- EIF-2alpha
- EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- EIF2S1
Background
EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.