gmh.2015.21

Wnt-5b Antibody Summary

    Immunogen
    Synthetic 14 amino acid peptide from internal region of human WNT5B.
    Epitope
    internal domain of human
    Specificity
    Human WNT5B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Species
    Monkey (100%), Primate (93%). Backed by our 100% Guarantee.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    WNT5B
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Immunohistochemistry-Paraffin 5 ug/ml
    Positive Control Wnt-5b Lysate (NBL1-17873)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Orangutan (100%), Gibbon (100%), Gorilla (93%), Marmoset (93%), Canine (86%), Elephant (86%), Equine (86%), Rabbit (86%), Opossum (86%).

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS
    Preservative
    0.1% Sodium Azide
    Concentration
    1.0 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for Wnt-5b Antibody

      MGC2648
      protein Wnt-5b
      wingless-type MMTV integration site family, member 5B
      WNT-5B protein
      Wnt5b
      Wnt-5b

Background

WNT5B is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt proteins constitute a large family of secreted molecules that are involved in intercellular signalling during development and have been implicated in oncogenesis. Very little is known about the structure of Wnts as they are notoriously insoluble; but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines. The Wnt proteins seem to adhere to the plasma membrane of the secreting cells and are therefore likely to signal over only few cell diameters. Fifteen major Wnt gene families have been identified in vertebrates, with multiple subtypes within some classes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

AF-354

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