Tau [p Thr231] Antibody (PHF-6) Summary

Paired helical filaments tau preparation from human brain.

p Thr231

pan-Neuronal Marker

Reacts with human Tau phosphorylated at threonine 231 and fetal tau. The antibody also reacts with dephosphorylated neurofibrillary tangles. MAB5450 is reactive with the Thr231 phosphorylated and diphosphorylated peptides. No reactivity with normal adult tau or with unphosphorylated or serine 235 phosphorylated protein.

IgG1

Monoclonal

Mouse

MAPT

Unpurified

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Western Blot: non-phosphate buffers recommended. Specific for phospho-tau however highly phosphorylated blocking materials like non-fat milk can sometimes cause difficulties, thus we generally recommend blocking western Blots with TBS-1-2% BSA solutions (filtered through a 0.45um membrane) for better results. Immunohistochemistry: fresh frozen tissues with Tris-NaCl-Triton treatment {http://www.jhc.org/cgi/content/full/48/12/1627} & http://ajp.amjpathol.org/cgi/content/full/160/6/2045

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Ascites

No Preservative

Unpurified