peerj.1444

Taf4 Antibody Summary

    Immunogen
    TAF4 (NP_003176 715 a.a. – 800 a.a.) partial recombinant protein with GST tag. LQPPVLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTPQQIQLNPLQPVPVVKPAVLPGT
    Specificity
    TAF4 – TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Mouse
    Gene
    TAF4
    Purity
    Unpurified
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Applications/Dilutions

    Dilutions
        Western Blot
        ELISA
    Application Notes
    The quality control of this antibody is limited to WB on the immunizing protein. It has been used for ELISA. Abnovas recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    Whole antisera with 50% Glycerol
    Preservative
    No Preservative
    Purity
    Unpurified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Taf4 Antibody

      FLJ41943
      TAF(II)130
      TAF(II)135
      TAF2C1TBP-associated factor 4
      TAF2CRNA polymerase II TBP-associated factor subunit C
      TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor
      TAF4A
      TAFII130TAFII-130
      TAFII135TAFII-135
      TATA box binding protein (TBP)-associated factor, 135kD
      TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD
      Transcription initiation factor TFIID 130 kDa subunit
      transcription initiation factor TFIID 135 kD subunit
      transcription initiation factor TFIID subunit 4

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

H-90 (dihydrochloride)

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