TLR4 Antibody (3C3) [FITC] Summary
CHO cells expressing chimeric human TLR4/MD-2 fusion protein
IgG1
Monoclonal
Mouse
TLR4
Protein A or G purified
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Applications/Dilutions
- Flow Cytometry 1:10-1:1000
- LPS from E. Coli, TLR4 ligand
- TLR4 Inhibitor Peptide Set
- TIRAP (TLR2 and TLR4) Inhibitor Peptide Set
Reactivity Notes
Human
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS and 1.0% BSA
0.05% Sodium Azide
Protein A or G purified
Alternate Names for TLR4 Antibody (3C3) [FITC]
- ARMD10
- CD284 antigen
- CD284
- hTollhomolog of Drosophila toll
- TLR4
- TOLL
- toll-like receptor 4
Background
The monoclonal antibody 3C3 reacts with human Toll-like receptor 4 (TLR4, CD284). TLRs belong to a family of proteins that specifically recognize and sense microbial products. They are highly conserved throughout evolution. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. TLRs identified as type I transmembrane signalling receptors act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. The monoclonal antibody 3C3 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.