TLR3 Antibody (TLR3.7) [PE] Summary
Ba/F3 cells transfected with human TLR3 (CD283).
NB100-66629 recognizes human and canine TLR3, otherwise known as CD283. TLR3 is a member of the Toll-like receptor (TLR) family and functions as a receptor for double-stranded (ds) RNA.TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. Cell surface expression of TLR3 has been reported on MRC-5 fibroblasts (1), and this antibody has been shown to suppress poly (I) : poly C mediated IFN beta production by these cells.
IgG1
Monoclonal
Mouse
TLR3
Protein A or G purified
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Applications/Dilutions
- Flow Cytometry 1:10-1:1000
This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- Polyinosinic-polycytidylic acid
Reactivity Notes
Human and Dog
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.2 mg/ml
Protein A or G purified
Alternate Names for TLR3 Antibody (TLR3.7) [PE]
- CD283 antigen
- CD283
- TLR3
- toll-like receptor 3
Background
The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-?B through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-B production by human fibroblasts naturally expressing TLR3 on their surface.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.