srep28332

TIMP-1 Antibody Summary

    Immunogen
    A synthetic peptide from the N terminal half of human TIMP1.
    Localization
    Cytoplasmic
    Specificity
    TIMP1
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    TIMP1
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        Immunohistochemistry 1:10-1:500
        Immunohistochemistry-Frozen 1:100
        Immunohistochemistry-Paraffin 1:10-1:500
    Application Notes
    This antibody can be used in Immunohistochemistry-paraffin. Staining of formalin fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Recommended Starting Dilutions*: IHC-FFPE: 1/100 Not tested in other applications. *Optimal dilutions should be determined by the end user.
    Positive Control Breast Lysate (NB820-59203)
TIMP-1 Lysate (NBL1-16924)
Bladder Lysate (NB820-59173)
Ovary Lysate (NB820-59442)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Buffer
    10mM PBS (pH 7.4) and BSA
    Preservative
    Sodium Azide
    Concentration
    0.2 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for TIMP-1 Antibody

      CLGI
      Collagenase inhibitor
      collagenase inhibitor)
      EPATIMP-1
      EPO
      erythroid potentiating activity
      Erythroid-potentiating activity
      Fibroblast collagenase inhibitor
      FLJ90373
      HCI
      metalloproteinase inhibitor 1
      TIMP metallopeptidase inhibitor 1
      TIMP1
      TIMP-1
      TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
      Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP-0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

product targets : BMX Kinase inhibitors

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