fmicb.2016.00671

TCR gamma/delta Antibody (GL-3/5E11) [Allophycocyanin] Summary

    Immunogen
    Mouse intraepithelial lymphocytes
    Specificity
    Detects mouse TCR gamma /δ.
    Source
    N/A
    Isotype
    IgG2 Kappa
    Clonality
    Monoclonal
    Host
    Hamster
    Gene
    Cd3d
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Applications/Dilutions

    Dilutions
        Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

    Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
    Buffer
    Supplied in a saline solution containing BSA and Sodium Azide.
    Preservative
    Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCR gamma/delta Antibody (GL-3/5E11) [Allophycocyanin]

      CD3d
      delta polypeptide
      T cell receptor gamma locus
      T3D
      T-cell antigen receptor, gamma polypeptide
      T-cell rearranging gene, gamma
      T-cell receptor, gamma cluster
      TCR gamma/delta
      TCRG
      TiT3
      TRG

Background

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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