Spectrin alpha 1 Antibody (B12G3) Summary
Native human beta spectrin
Cytoplasmic surface of the cell membrane
Recognises alpha (240 kD) and beta (220 kD) subunits of human spectrin.
IgM
Monoclonal
Mouse
SPTA1
IgM purified
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Applications/Dilutions
- ELISA 1:100-1:2000
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
Reactivity Notes
Human. Not yet tested in other species.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS
0.09% Sodium Azide
6.5 mg/ml
IgM purified
Alternate Names for Spectrin alpha 1 Antibody (B12G3)
- alpha-I spectrin
- EL2
- erythrocyte
- HS3
- spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
Background
Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.