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Serpin A6/Cortisol Binding Globulin Antibody Summary

    Immunogen
    Synthetic peptide C-DLFTNQANFSRIT from an internal region of human SERPINA6 / CBG (NP_001747.2).
    Specificity
    Human SERPINA6 / CBG.
    Predicted Species
    Primate (92%), Monkey (92%). Backed by our 100% Guarantee.
    Isotype
    IgG
    Clonality
    Polyclonal
    Host
    Goat
    Gene
    SERPINA6
    Purity
    Immunogen affinity purified
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Applications/Dilutions

    Dilutions
        ELISA 1:100 – 1:2000
        Immunohistochemistry-Paraffin 3-6 ug/ml
    Application Notes
    IHC: NBP1-52396 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Orangutan (100%), Gibbon (100%), Marmoset (92%), Bat (85%), Rabbit (85%).

Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    TBS (pH 7.3) and 0.5% BSA
    Preservative
    0.02% Sodium Azide
    Concentration
    0.5 mg/ml
    Purity
    Immunogen affinity purified

Alternate Names for Serpin A6/Cortisol Binding Globulin Antibody

      antitrypsin), member 6
      CBG
      CBGcorticosteroid-binding globulin
      corticosteroid binding globulin
      member 6
      serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
      Serpin A6
      serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
      transcortin

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

GSK2334471

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