1354750X.2015.1130191

Recombinant Rat CCL2/MCP1 Protein Summary

    Description
    Recombinant bioactive protein containing 125 amino acids for Rat CCL2/MCP1

    Source:E. coli
    Amino Acid Sequence:(P14844)

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    Determined by its ability to chemoattract human monocytes using a concentration range of 10-100 ng/ml.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    CCL2
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    14 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Sterile filtered and then lyophilized with no additives. The protein may appear as a haze or film which is difficult to see at the bottom of the vial.
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other buffered solutions and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Rat CCL2/MCP1 Protein

      C-C motif chemokine 2
      chemokine (C-C motif) ligand 2
      GDCF-2
      HC11Monocyte secretory protein JE
      MCAFMonocyte chemotactic and activating factor
      MCP1 Monocyte chemotactic protein 1
      MCP-1 Small-inducible cytokine A2
      MGC9434
      Monocyte chemoattractant protein 1
      monocyte chemoattractant protein-1
      SCYA2HSMCR30
      small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
      small inducible cytokine subfamily A (Cys-Cys), member 2
      SMC-CF

Background

Macrophage/monocyte chemoattractant protein-1 (MCP-1) plays an important role in the inflammatory response of blood monocytes and tissue microphages. Recombinant rat MCP-1 is a 14.0 kDa protein containing 125 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

LY2784545

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