Recombinant Mouse PDGFA Protein Summary
Recombinant non-glycosylated homodimer of 126 amino acids corresponding to PDGF.
Source: E. coli
This reagent has been tested in bioassays using the mouse cell line 3T3. The ED50 measured in a 3T3 proliferation assay is typically 10 ng/ml, corresponding to a specific activity of approximately 1 x10^5Units/mg.
E. coli
Biologically Active Protein
PDGFA
>95% pure by SDS-PAGE
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Applications/Dilutions
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Phosphate buffered saline, pH 7.2
0.09% Sodium Azide
0.1 mg/ml
>95% pure by SDS-PAGE
Alternate Names for Recombinant Mouse PDGFA Protein
- PDGF-1
- PDGF1PDGF A-chain
- PDGF-A
- Platelet-derived growth factor A chain
- platelet-derived growth factor alpha chain
- platelet-derived growth factor alpha isoform 2 preproprotein
- platelet-derived growth factor alpha polypeptidePDGF subunit A
- platelet-derived growth factor subunit A
Background
The platelet-derived growth factor AA (PDGF-AA) is a member of the PDGF family. The PDGF proteins are derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB). PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.