s12955-015-0398-y

Recombinant Mouse IL25 Protein Summary

    Description
    Recombinant protein containing 143 amino acids for Mouse IL25

    Source:E. coli
    Amino Acid Sequence:(Q9CPT4) – MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL

    Preparation
    Method
    >97% SDS-PAGE
    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    C19orf10
    Purity
    >95%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    15.8 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized from a sterile filtered solution containing PBS pH 7.4
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in sterile ddH2O to a concentration> 100 ug/ml. This solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Mouse IL25 Protein

      C19orf10
      chromosome 19 open reading frame 10
      EUROIMAGE1875335
      IL-25
      IL25IL27w
      IL27
      IL-27
      interleukin 25
      interleukin 27 working designation
      Interleukin-25
      Ly6elg
      MYDGF
      R33729_1
      SF20
      SF20hypothetical protein LOC56005
      Stromal cell-derived growth factor SF20
      stromal cell-derived growth factor

Background

Mouse SF20 is a bone marrow stroma-derived growth factor. SF20 is expressed in the bone marrow, spleen stroma cells, resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells. SF20 has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Among SF20s biological activities is stimulation of the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells. Chromosome 19 Open Reading Frame 10 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 15.8kDa. The Mouse SF20 is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

AMG 901

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