journal.pone.0145306

Recombinant Mouse IL-17F Protein Summary

    Description
    Recombinant bioactive protein containing 266 amino acids for Mouse IL-17F

    Source:E. coli
    Amino Acid Sequence:(Q7TNI7)

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependent induction of IL-6 in NHDF is 1.94 ng/ml.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    IL17F
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    29.8 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized with no additives
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in sterile H2O to a concentration of 0.1 mg/ml (Note: Gently pipetting to dissolve). The solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Mouse IL-17F Protein

      Cytokine ML-1
      IL17F
      IL-17F
      IL-17Finterleukin-17F
      IL24
      IL-24
      interleukin 17F
      Interleukin-24
      ML-1
      ML1interleukin-24

Background

Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activated CD4+ T cells have also been shown to produce Mouse IL-17F. Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A. Recombinant Mouse IL-17F produced in E.Coli is a non-glycosylated polypeptide comprised of two monomeric subunit each of mIL-17F. The dimer consists of 266 amino acids, with an approximate molecular weight of 29.8 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

WP1131

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