Recombinant Mouse IFN-gamma Protein Summary
A Partial Recombinant Proteins to IFN-gamma.
Method
This product isE. coli expressed amino acids His 23-Cys 155 of mouse IFN- (accession # NM_008337).
This reagent has been tested in bioassays using the mouse fibroblast cell line L929. The ED50 measured in a L929 protection is typically 100 pg/ml, corresponding to a specific activity of approximately 1×10^7Units/mg.
Partial Recombinant Protein
IFNG
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Applications/Dilutions
Reactivity Notes
This protein was made to the mouse sequence.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Sterile 10 mM NaH2PO4, 150 mM NaCl, 1 mM DTT, with no carriers. 0.22 um filtered.
No Preservative
Notes
Purity: Greater than 98%, as determined by SDS-PAGE
Alternate Names for Recombinant Mouse IFN-gamma Protein
- IFG
- IFI
- IFNG
- IFNgamma
- IFN-gamma
- Immune interferon
- interferon gamma
- interferon, gamma
Background
Mouse IFN-gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.