s13071-015-1266-7

Recombinant Mouse EGF Protein Summary

    Description
    Recombinant bioactive protein containing 53 amino acids for Mouse EGF

    Source:E. coli
    Amino Acid Sequence:(P01132 )

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    EGF
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    6 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Lyophilized with no additives
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Mouse EGF Protein

      beta-urogastrone
      EGF
      epidermal growth factor (beta-urogastrone)
      epidermal growth factor
      HOMG4
      pro-epidermal growth factor
      URG
      Urogastrone

Background

EGF (Epidermal Growth Factor) is polypeptide growth factor that stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.0 kDa protein containing 53 amino acid residues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

MG-133

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