fpls.2015.01132

Recombinant Human hCG Alpha (Intact) Protein Summary

    Description
    Native bioactive protein containing amino acids for Human hCG Alpha (Intact)

    Source:Urine of post-menopausal women

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    hCG is biologically active compared to standard. The biological activity of hCG is determined by using the seminal vesicle weight gain test in male rats described in the “Chorionic Gonadotrophins” monograph of the European Pharmacopoeia. The in vivo biological activity of hCG has been calibrated against the third international reference preparation IS75/587 for chorionic gonadotropin. The specific activity was found to be 5000 IU/mg.
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    CGA
    Purity
    >95%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    36.7 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Sterile filtered and lyophilized with no additives
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml.

Alternate Names for Recombinant Human hCG Alpha (Intact) Protein

      glycoprotein hormones alpha chain
      CG-ALPHA
      FSHA
      glycoprotein hormones, alpha polypeptide
      GPHa
      GPHA1
      HCG
      LHA
      TSHA

Background

s hCG is biologically active compared to standard. The biological activity of hCG is determined by using the seminal vesicle weight gain test in male rats described in the “Chorionic Gonadotrophins” monograph of the European Pharmacopoeia. The in vivo biological activity of hCG has been calibrated against the third international reference preparation IS75/587 for chorionic gonadotropin. The specific activity was found to be 5000 IU/mg.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

LY2157300

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