Recombinant Human beta-NGF Protein Summary
Recombinant bioactive protein for Human beta-NGF
Source:CHO cells
Amino Acid Sequence:(P01138)
Method
>98% SDS-PAGE
The ED50 as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.
CHO
Biologically Active Protein
NGF
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
16.95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Sterile filtered and then lyophilized in 30 mM Sodium Acetate, pH 5.5 containing 1% HAS.
Lyoph
>95%, by SDS-PAGE
Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Alternate Names for Recombinant Human beta-NGF Protein
- Beta nerve growth factor precursor
- beta-nerve growth factor
- betaNGF
- beta-NGF
- HSAN5
- MGC161426
- nerve growth factor (beta polypeptide)
- nerve growth factor, beta subunit
- NGFB
- NGFBMGC161428
Background
Nerve growth factor-Beta (NGF-Beta ) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-Beta is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.