rsos.150432

Recombinant Human TNF RI/TNFRSF1A Protein Summary

    Description
    Recombinant bioactive protein containing 162 amino acids for Human TNF RI/TNFRSF1A

    Source:E. coli
    Amino Acid Sequence:(AAA36756)

    Preparation
    Method
    >98% SDS-PAGE
    Details of Functionality
    The biological activity of sTNF-RI was determined by its inhibitory effect of the TNF-a mediated cytotoxicity in murine L-929 cells. The ED50 for the effect in the presence of 0.25 ng/ml of recombinant TNF-a, is 0.05 mg/ml.
    Source
    E. coli
    Protein/Peptide Type
    Biologically Active Protein
    Gene
    TNFRSF1A
    Purity
    >95%, by SDS-PAGE
    Endotoxin Note
    <0.1 ng/ug

Applications/Dilutions

    Theoretical MW
    18.3 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at -20 to -80C. Avoid freeze-thaw cycles.
    Buffer
    Sterile filtered and lyophilized in 5 mM Tris pH 8.0
    Concentration
    Lyoph
    Purity
    >95%, by SDS-PAGE
    Reconstitution Instructions
    Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Alternate Names for Recombinant Human TNF RI/TNFRSF1A Protein

      CD120a antigen
      CD120a
      FPF
      p55
      p55-R
      p60
      TNF RI
      TNFARMGC19588
      TNF-R
      TNF-R1
      TNFR1TBP1
      TNFR55
      TNF-R55
      TNFR60
      TNFRI
      TNF-RI
      TNF-R-I
      TNFR-I
      TNFRSF1A
      tumor necrosis factor binding protein 1
      Tumor necrosis factor receptor 1
      tumor necrosis factor receptor 1A isoform beta
      tumor necrosis factor receptor superfamily member 1A
      tumor necrosis factor receptor superfamily, member 1A
      tumor necrosis factor receptor type 1
      Tumor necrosis factor receptor type I
      tumor necrosis factor-alpha receptor

Background

Tumor Necrosis Factor receptor type I (TNF-RI) is also referred as p60. The p60 form of the receptor is primarily expressed on epithelial cells. Recombinant Human soluble TNF receptor Type I (sTNF-RI) is a 18.3 kDa protein containing 162 amino acid residues comprising the extra Cellular domain of TNF Receptor Type I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

EGF817

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