Recombinant Human TNF RII/TNFRSF1B Protein Summary
Recombinant bioactive protein for Human TNF RII/TNFRSF1B
Source:E. coli
Amino Acid Sequence:(P20333) – MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.
Method
>97% SDS-PAGE
The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2 ug/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-a.
E. coli
Biologically Active Protein
TNFRSF1B
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
TNFR2 was Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Lyoph
>95%, by SDS-PAGE
It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Alternate Names for Recombinant Human TNF RII/TNFRSF1B Protein
- CD120b antigen
- CD120b
- Etanercept
- p75 TNF receptor
- p75TBPII
- p75TNFR
- soluble TNFR1B variant 1
- TNF RII
- TNFBRp80 TNF-alpha receptor
- TNF-R2
- TNFR2TNFR1B
- TNF-R75
- TNFR80
- TNFRII
- TNF-RII
- TNF-R-II
- TNFR-II
- TNFRSF1B
- tumor necrosis factor beta receptor
- tumor necrosis factor binding protein 2
- Tumor necrosis factor receptor 2
- tumor necrosis factor receptor superfamily member 1B
- tumor necrosis factor receptor superfamily, member 1B
- Tumor necrosis factor receptor type II
Background
Tumor Necrosis Factor Receptor Type II is an inhibitor of TNF and can block TNF bioactivity. Recombinant human TNF-RII produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extraCellular ligand-binding portion of the human 75 kilodalton (p75) tumor necrosis factor receptor (TNFR) linked to the Fc portion of human IgG1. The Fc component of TNFRII contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilodaltons.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.