Recombinant Human TIGAR/C12orf5 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-270 of Human TIGAR/C12orf5
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR
E. coli
Recombinant Protein
C12ORF5
>90%, by SDS-PAGE
Applications/Dilutions
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
No Preservative
0.5 mg/ml
>90%, by SDS-PAGE
Alternate Names for Recombinant Human TIGAR/C12orf5 Protein
- C12orf5
- chromosome 12 open reading frame 5
- EC 3.1.3.46
- probable fructose-2,6-bisphosphatase TIGAR
- TIGAR
- TIGARTP53-induced glycolysis and apoptosis regulator
- transactivated by NS3TP2 protein
Background
TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets :　Adenosine Deaminase inhibitors