PRBM.S101040

Recombinant Human TIFA Protein Summary

    Description
    A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-184 of Human TIFA

    Source: E.coli

    Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTSFED ADTEETVTCL QMTVYHPGQL QCGIFQSISF NREKLPSSEV VKFGRNSNIC HYTFQDKQVS RVQFSLQLFK KFNSSVLSFE IKNMSKKTNL IVDSRELGYL NKMDLPYRCM VRFGEYQFLM EKEDGESLEF FETQFILSPR SLLQENNWPP HRPIPEYGTY SLCSSQSSSP TEMDENES

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    TIFA
    Purity
    >90%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    24 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
    Preservative
    No Preservative
    Concentration
    0.5 mg/ml
    Purity
    >90%, by SDS-PAGE

Alternate Names for Recombinant Human TIFA Protein

      Putative MAPK-activating protein PM14
      Putative NF-kappa-B-activating protein 20
      T2BPMGC20791
      T6BP
      TIFAA
      TRAF2 binding protein
      TRAF2-binding protein
      TRAF6 binding protein
      TRAF-interacting protein with a forkhead-associated domain
      TRAF-interacting protein with FHA domain-containing protein A
      TRAF-interacting protein with forkhead-associated domain

Background

TIFA, also known as TRAF-interacting protein with FHA domain-containing protein A, is an adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. This protein induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. Recombinant human TIFA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : RGS Protein inhibitors

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