Recombinant Human Serpin A12 Protein Summary
Recombinant bioactive protein containing 415 amino acids for Human Serpin A12
Source:E. coli
Amino Acid Sequence:(Q8IW75)
Method
>98% SDS-PAGE
E. coli
Biologically Active Protein
SERPINA12
>95%, by SDS-PAGE
<0.1 ng/ug
Applications/Dilutions
Unit Definition: > 1 unit/ug. One unit of Turbo3C (HRV3C) Protease cleaves >95% of 100ug of target protein at 4C for 16 hours
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from 50 mM Tris, pH 7.5 + 150 mM NaCl.
1 mg/ml
>95%, by SDS-PAGE
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Alternate Names for Recombinant Human Serpin A12 Protein
- antitrypsin), member 12
- member 12
- OL-64
- OL-64Visceral adipose-specific serpin
- serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
- Serpin A12
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
- Vaspin
- Visceral adipose tissue-derived serine protease inhibitor
Background
Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 415 amino acids and having a molecular mass of 47 kDa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.