journal.pone.0151278

Recombinant Human RAB18 Protein Summary

    Description
    A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-206 of Human RAB18

    Source: E.coli

    Amino Acid Sequence: MASMTGGQQM GRGSHMDEDV LTTLKILIIG ESGVGKSSLL LRFTDDTFDP ELAATIGVDF KVKTISVDGN KAKLAIWDTA GQERFRTLTP SYYRGAQGVI LVYDVTRRDT FVKLDNWLNE LETYCTRNDI VNMLVGNKID KENREVDRNE GLKFARKHSM LFIEASAKTC DGVQCAFEEL VEKIIQTPGL WESENQNKGV KLSHREEGQG GGACGGYCSV L

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    RAB18
    Purity
    >90%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    24.5 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT
    Preservative
    No Preservative
    Concentration
    1 mg/ml
    Purity
    >90%, by SDS-PAGE

Alternate Names for Recombinant Human RAB18 Protein

      RAB18 small GTPase
      RAB18, member RAS oncogene family
      RAB18LI1
      ras-related protein Rab-18

Background

RAB18 is a member of a family of Ras-related small GTPase that is lipid-anchored to the cytoplasmic side of the cell membrane. It plays a role in apical recycling and endocytosis and is also thought to be involved in protein transport between early endosomes and the plasma membrane. Recombinant human Rab18 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : P-glycoprotein inhibitors

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