Recombinant Human PTRH1 Protein Summary
A recombinant protein corresponding to the first five N-terminal of PTRH1.
Source: E. coli
Amino Acid Sequence: Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe
rHuPTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0 x 10^4 Units/mg.
E. coli
Biologically Active Protein
PTRH1
> 98 % pure by SDS-PAGE and HPLC
Less than 0.1 ng/ug (IEU/ug) of rHuPTH.
Applications/Dilutions
4.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Lyophilized after extensive dialyses against sodium citrate containing sodium chloride, citric acid, EDTA-Na2, Tween 80 and Mannitol.
No Preservative
LYOPH
> 98 % pure by SDS-PAGE and HPLC
Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.
Notes
After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Alternate Names for Recombinant Human PTRH1 Protein
- C9orf115
- chromosome 9 open reading frame 115
- EC 3.1.1.29
- MGC51999
- peptidyl-tRNA hydrolase 1 homolog (S. cerevisiae)
- probable peptidyl-tRNA hydrolase
- PTH
- PTH1
Background
Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calciumin the blood, whereas calcitonin (a hormone produced by the parafollicular cells of the thyroid gland) acts to decrease calcium concentration. PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptorin three parts of the body: In the bones- It enhances the release of calcium from the large reservoir contained in the bones. Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH. Stimulation is indirect since osteoclasts do not have a receptor for PTH; rather, PTH binds to osteoblasts, the cells responsible for creating bone. Binding stimulates osteoblasts to increase their expression of RANKL, which can bind to osteoclast precursors containing RANK, a receptor for RANKL. The binding of RANKL to RANK stimulates these precursors to fuse, forming new osteoclasts which ultimately enhances the resorption of bone.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.