Recombinant Human PMM1/Phosphomannomutase 1 Protein Summary
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-262 of Human PMM1/Phosphomannomutase 1
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA
E. coli
Recombinant Protein
PMM1
>90%, by SDS-PAGE
Applications/Dilutions
31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl, 0.1mM PMSF
No Preservative
0.5 mg/ml
>90%, by SDS-PAGE
Alternate Names for Recombinant Human PMM1/Phosphomannomutase 1 Protein
- brain glucose-1,6-bisphosphatase
- EC 5.4.2.8
- phosphomannomutase 1
- PMM 1
- PMMH22
- PMMH-22
- Sec53
Background
PMM1 (Phosphomannomutase 1) is an enzyme that involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. This enzyme catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. In addition, it may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain. Recombinant human PMM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
product targets : EBI2_GPR190 inhibitors