reveh-2016-0010

Recombinant Human NECAP2 Protein Summary

    Description
    A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-263 of Human NECAP2

    Source: E.coli

    Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    NECAP2
    Purity
    >85%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    30.5 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
    Preservative
    No Preservative
    Concentration
    1 mg/ml
    Purity
    >85%, by SDS-PAGE

Alternate Names for Recombinant Human NECAP2 Protein

      adaptin ear-binding coat-associated protein 2
      adaptin-ear-binding coat-associated protein 2
      FLJ10420
      NECAP endocytosis associated 2
      NECAP endocytosis-associated protein 2
      NECAP-2

Background

NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis. Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : 12-Lipoxygenase inhibitors

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