journal.pone.0151387

Recombinant Human NCALD Protein Summary

    Description
    A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-193 of Human NCALD

    Source: E.coli

    Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF

    Source
    E. coli
    Protein/Peptide Type
    Recombinant Protein
    Gene
    NCALD
    Purity
    >90%, by SDS-PAGE

Applications/Dilutions

    Theoretical MW
    24.4 kDa.
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 100mM NaCl
    Preservative
    No Preservative
    Concentration
    0.5 mg/ml
    Purity
    >90%, by SDS-PAGE

Alternate Names for Recombinant Human NCALD Protein

      MGC33870
      MGC74858
      neurocalcin delta
      neurocalcin-delta

Background

Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

product targets : PDK-8 inhibitors

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